Everything You Need to Know About Reconstituting / Mixing Peptides
Quick tool for the lab:
Use our Peptide calculator to convert mg↔mL and mcg↔IU and determine concentration points.
Peptide Storage: Preserving Quality and Stability
Proper storage is vital for maintaining the stability and integrity of peptides. Peptides in their lyophilised (freeze-dried) form are safe for transport as they are protected from UV light and remain stable in a freeze-dried state. However, we recommend refrigerating them as soon as possible upon arrival to ensure proper storage and extend their longevity.
At US Peptides, we take storage seriously. We maintain peptides in a controlled environment with a temperature-controlled walk-in freezer and three medical fridges, all closely monitored for optimal temperature stability. Peptides stored at -20°C in a freezer will remain stable for up to 48 months, making this ideal for long-term storage.
Storage Duration Guidelines
| Type of Peptide | Lyophilised Storage | Reconstituted Storage |
|---|---|---|
| Basic Peptides | Up to 48 months at -20°C | 2 – 8 weeks at 2-8°C |
| Acidic Peptides | Up to 48 months at -20°C | 2 – 8 weeks at 2-8°C |
| Neutral Peptides | Up to 48 months at -20°C | 2 – 8 weeks at 2-8°C |
| Hydrophobic Peptides | Up to 48 months at -20°C | 2 – 8 weeks at 2-8°C |
| Hydrophilic Peptides | Up to 48 months at -20°C | 2 – 8 weeks at 2-8°C |
Peptide Reconstitution: Achieving Optimal Results
Equalising Pressure in the Vial
When reconstituting, it's essential to equalise the pressure in the vial. Due to the drying process, some vials may have a vacuum, while others could have positive pressure. To balance this, insert a sterile needle that's half-filled with air into the vial's rubber stopper.
Choosing the Right Solvent
Bacteriostatic water is commonly used for reconstitution, although some peptides may require acetic acid for complete dissolution. For hydrophobic peptides, a 50% aqueous acetic acid solution can be helpful.
Avoid Excessive Solution Volumes
Adding too much solution can fragment and break down peptides. Use between 1 to 2.5 ml of solution for reconstitution unless specific studies confirm that a higher volume is safe.